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91.
E. Arseniuk 《Plant Breeding》1989,103(4):310-318
The effect of induced autotetraploidy on the response of red clover to Sclerotinia clover rot (SCR) was studied under laboratory, glasshouse and field conditions. To assess the response in the laboratory, the detached leaf technique was employed, whereas in the glasshouse and in the field, 6—14 weeks old seedlings and adult plants, respectively, were artificially inoculated with the pathogen grain inoculum. The results of the studies showed that great genotypic variability occurs within diploid (2×)and tetraploid (4×)red clover in response to that disease. Generally, tetraploid red clover, in comparison to diploid, is more resistant to SCR. The laboratory, glasshouse and field experiments showed that the effect of induced autotetraploidy on SCR resistance is positive. The hardening of plant with light and temperature enlarged the differences in reponse to SCR between ploidy levels, family pairs and individual strains with every testing method. Other factors, like plant age and flowering time, influencing red clover response to SCR are also discussed. 相似文献
92.
Sclerotinia sclerotiorum (Lib.) de Bary is a major limiting factor for sunflower (Helianthus annuus) production. This study was conducted to (1) evaluate a large number of current sunflower lines for their reaction to Sclerotinia, and (2) determine the optimum allocation of resources for their selection. A set of 85 inbred lines was screened under artificial leaf infection in three environments. The test revealed ample variation for all resistance traits in the current germplasm. A line selected from NDBLOS, an oilseed sunflower germplasm poo, and its derivatives were most resistant to Sclerotinia. Lines derived from interspecific crosses with the wild species Helianthus tuberosus and Helianthus argophyllus also showed satisfactory Sclerotinia resistance. The evaluation of five plants per plot in two replications and four environments appears to be a reasonable allocation of resources in order to optimize the selection response. 相似文献
93.
Dothistroma needle blight (DNB) is a serious needle disease of conifers that primarily affects pine species (Pinus spp.). Dothistroma septosporum is one of the DNB pathogens that has a diverse range of host species excluding Pinus armandii. In 15 inoculated P. armandii seedlings, D. septosporum acervuli were observed in 43 infected needles of ten seedlings with a mean disease severity of 1.11% at 25 weeks after inoculations, demonstrating the potential of D. septosporum to cause symptoms on the needles of P. armandii via artificial inoculation. The disease severity of P. armandii was similar to the positive control, Pinus nigra (median 0.75 for P. armandii to 0.70 for P. nigra), thus, P. armandii acts under artificial conditions as a susceptible host species. 相似文献
94.
以铁皮石斛商品瓶苗和盆栽苗茎段为试材,添加不同浓度6-BA、NAA,诱导茎段腋芽、原球茎和不定根,建立再生体系,快速繁殖铁皮石斛组培苗,以期为秦岭淮河一线以北地区人工繁殖铁皮石斛提供参考依据。结果表明:外植体宜采用70%酒精浸泡20 s,0.1%升汞浸泡8 min消毒;MS+6-BA 3.0~5.0 mg·L^-1+NAA 0.1~1.0 mg·L^-1适合腋芽分化和增殖,分化率92%~100%;1/2MS+6-BA 2.0 mg·L^-1+NAA 0.5 mg·L^-1+土豆泥15%适合原球茎诱导增殖,增殖倍数7.7;1/2MS+NAA 0.3 mg·L^-1生根率为100%;以松针土移栽,成活率83%。 相似文献
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以‘霍巴’海棠(Malus‘Hopa’)的茎尖和带腋芽的幼嫩茎段为外植体,建立了比较高效的‘霍巴’海棠组培快繁体系。结果表明:‘霍巴’海棠茎尖及茎段外植体在MS+6-BA0.1mg/L+IAA 0.2mg/L+3%蔗糖+0.48%琼脂(pH 5.8)培养基上生长状况较好;将其组培苗在WPM+6-BA 0.5mg/L+NAA 0.1mg/L+ZT 0.01mg/L+3%蔗糖+0.48%琼脂(pH 5.8)培养基上继代培养40d左右,继代增殖系数均可达到3.0左右;再经1/2 WPM+NAA0.8mg/L+1.5%蔗糖+0.48%琼脂(pH 5.8)培养基生根壮苗后,移栽成活率可达97.5%。 相似文献
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